Comparative Transcriptome Analysis Provides New Insights into the Molecular Regulatory Mechanism of Adventitious Root Formation in Ramie (Boehmeria nivea L.)

The occurrence of adventitious roots is necessary for the survival cuttings. In this study, comparative transcriptome analysis between two ramie (Boehmeria nivea L.) varieties with different root (AR) patterns was performed by mRNA-Seq before rooting (control, CK) and 10 days water-induced (treatment, T) to reveal regulatory mechanism rooting. Characterization cultivars, Zhongzhu No 2 (Z2) Huazhu 4 (H4), indicated that Z2 had a high rate but H4 low rate. Twelve cDNA libraries were constructed, total 26,723 genes expressed. non-water culture condition, number distinctive in 2.7 times Z2, while water nearly H4. A 4411 5195 differentially expressed (DEGs) identified comparison H4CK vs. H4T Z2CK Z2T, respectively. After culture, more DEGs upregulated downregulated Gene ontology (GO) functional polysaccharide metabolic process, carbohydrate cellular cell wall macromolecule photosystem GO terms distinctively significantly enriched Simultaneously, Kyoto Encyclopedia Genes Genomes (KEGG) showed photosynthesis, photosynthesis antenna proteins, starch sucrose metabolism pathways Moreover, KEGG jasmonic acid (JA) could interact ethylene regulate AR Z2. This study reveals transcriptomic divergence rates, provides insights into molecular formation ramie.